TELL ME FAST™ Mycobacterium Tuberculosis IgG/IgM Test Device (Serum/Plasma) is a qualitative test for the detection of IgG and IgM antibodies to M. Tuberculosis (TB) in human serum or plasma. The test provides a differential detection of anti-TB-IgG and anti-TB-IgM antibodies and can be used for the presumptive distinction between a primary and secondary TB infection. Serum or plasma samples may be used with this test. First a specimen is dispensed with sample buffer, the Gold antigen conjugate will bind to anti-TB IgG and IgM antibodies in the specimen sample which in turn will bind with Anti-Human IgG and Anti-Human IgM coated on the membrane as two separate lines in the test region as the reagent move across the membrane. The anti-Human antibodies on the membrane will bind the IgG or IgM antigen complex at the relevant IgG and or IgM test lines causing pale or dark colored lines to form at the IgG or IgM region of the test membrane. The intensity of the lines will vary depending upon the amount of antibody present in the sample. The appearance of colored line in a specific test region (IgG or IgM) should be considered as positive for that particular antibody type (IgG or IgM).
